The Short-term Effectiveness of Three Manipulations for Atlantoaxial Joint Disorders and the Effects on Surface Electromyography of Sternocleidomastoid Muscle / 中医杂志

ObjectiveTo compare the short-term effectiveness of the three different manipulations for atlantoaxial joint disorders and their effects on surface electromyography of sternocleidomastoid muscle. MethodsNinty patients with atlantoaxial joint disorders were randomly divided into the tendon relaxing manipulation group， the tendon relaxing plus rehabilitation manipulation group， and the conventional manipulation group， with 30 cases in each group， and each group of patients received the corresponding manipulation treatment for 2 weeks. The changes of visual analogue score （VAS） of occipital neck pain， evaluation scale for cervical vertigo （ESCV）， and averaged electromyography （AEMG） of surface electromyography of bilateral sternocleidomastoid muscles before and after the treatment were observed， and the clinical effectiveness and safety of the patients were compared among groups. ResultsThe VAS scores of patients in each group decreased， and the ESCV scores increased after treatment （P＜0.01）， and the tendon relaxing manipulation group and the tendon relaxing plus rehabilitation manipulation group were significantly better than the conventional manipulation group （P＜0.01）. The AEMG of the bilateral sternocleidomastoid muscles of the three groups increased after treatment （P＜0.01）； when compared among the three groups， the AEMG of the bilateral sternocleidomastoid muscles of the tendon relaxing plus rehabilitation manipulation group was higher than that of the tendon relaxing manipulation group， and the tendon relaxing manipulation group was higher than that of the conventional manipulation group （P＜0.05 or P＜0.01）. The cure and markedly effective rates of the tendon relaxing manipulation group， the tendon relaxing plus rehabilitation manipulation group， and the conventional manipulation group were 56.67%， 86.67%， and 36.67% respectively， showing statistically difference （K=10.21， P＜0.01）. ConclusionThe tendon relaxing manipulation and tendon relaxing plus rehabilitation manipulation can effectively improve the symptoms of vertigo， headache， and neck pain for patients with atlantoaxial joint disorders， and can improve the contraction function of sternocleidomastoid muscle， whose effectiveness are better than that of conventional manipulation.

Role and mechanism of circular RNA-vimentin in the proliferation and apoptosis of colorectal cancer cells / 中华消化杂志

Objective To investigate the role and mechanism of circular RNA-vimentin (circ-VIM) in the proliferation and apoptosis of colorectal cancer cells.Methods From December 2016 to December 2017, at Department of General Surgery of The First Affiliated Hospital of Zhengzhou University, the clinical data of 100 patients who underwent radical resection of colorectal cancer and were confirmed by pathological examination after operation were collected.The tumor tissues and corresponding paracancerous tissues (negative control) were also collected.The expression of circ-VIM in the colorectal cancer tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR).The proliferation of HCT-116 and HT29 colorectal cancer cells was detected by cell counting kit-8 assay.The ratio of apoptosis of HCT-116 and HT29 cells was measured by annexin Ⅴ/propidium iodide double staining assay.The mitochondrial membrane potential of HCT-116 and HT29 cells was examined by 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide (JC-1) assay.The expression changes of protein kinase B and mammalian target of rapamycin were tested by Western blotting.The target miRNA of circ-VIM was predicted by miRDB software.T-test and chi-square test were performed for statistical analysis. Results The expression of circ-VIM in colorectal cancer tissues was 2.387 ±0.536, which was higher than that in corresponding paracancerous tissues (1.110 ±0.134), and the difference was statistically significant (t =23.096, P <0.01).And the expression levels of circ-VIM were significantly different in patients with different tumor size, TNM stage and lymph node metastasis (all P <0.05).The proliferation of HCT-116 cells and HT29 cells in lenti-circ-VIM group was 0.737 ±0.023 and 0.835 ±0.025, respectively, which were both higher than those in control group (0.449 ±0.020 and 0.531 ±0.019), and the differences were statistically significant (t =20.706 and-15.374, both P <0.01).The proliferation of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was 0.236 ±0.027 and 0.243 ±0.019, which were lower than those in control group, and the differences were statistically significant (t =24.557 and -23.197, both P <0.01).The ratio of apoptosis of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was (18.00 ±1.82)％ and (20.80 ±0.61)％, which was higher than those in control group ((6.64 ±2.01)％ and (7.35 ±1.36)％), and the differences were statistically significant (t =8.826 and 17.454, both P <0.01).The fluorescence intensity ratio of JC-1 aggregate and JC-1 monomer of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was 2.21 ±0.12 and 1.40 ±0.11, which was lower than those in control group (14.54 ±1.00 and 9.24 ±1.18), and the differences were statistically significant (t =-19.558 and-15.685, both P <0.01), which indicated mitochondrial membrane potential decreased.After treated with lenti-circ-VIM-shRNA, the expression of phosphorylated protein kinase B, phosphorylated mammalian target of rapamycin, B-cell lymphoma-2 and mitochondrial cytochrome C at protein level were all down-regulated, however the expression of cytoplasmic cytochrome C, B-cell lymphoma-2 associated X protein and cleaved caspase-3 at protein level were all up-regulated.When the expression of circ-VIM was up-regulated, the expression of miRNA-147b, miRNA-4447 and miRNA-3656 was down-regulated.When the expression of circ-VIM was down-regulated, the expression of miRNA-147b, miRNA-4447 and miRNA-3656 was up-regulated.Conclusion The expression of circ-VIM in colorectal cancer is abnormally increased, which is involved in the proliferation and apoptosis of colorectal cancer cells.

Prenatal diagnosis of mosaic trisomy 2: a case report / 中华围产医学杂志

We reported a case of mosaic trisomy 2.The patient was a 29-year-old gravida who underwent amniocentesis at 20 weeks of gestation because of high risk of trisomy-21 in the first trimester screening.The test result revealed a karyotype of 47,XN,+2[10]/46,XX[40].At 26 gestational weeks,the fetus was found severe fetal growth restriction and oligohydramnios which was considered to be at risk of mosaic trisomy 2.The pregnancy was terminated at 27+ gestational weeks.The fetus had obviously abnormal appearances,including dolichocephaly,low-set ears,and micromandible.Autopsy was not performed due to the parents' refusal.

Chest pain during pregnancy: a clinical analysis of13 cases / 中华围产医学杂志

ObjectiveTo investigate the clinical feature, management, outcome and prognosis of chest pain during pregnancy.MethodsClinical data of 13 gravidas with chest pain admitted to the Fujian Medical University Union Hospital from January, 2013 to December, 2017 were analyzed retrospectively.Results Among the 13 cases, nine had acute chest pain (happen within 72 hours); two had chronic chest pain lasting over one month, and the other two had paroxysmal subacute chest pain (72 h to less than 30 d). Seven cases were complicated by cardiogenic disease/death including six with aortic dissection (patients No.1-6) and one with myocardial infarction (patient No.7). The remaining six cases were complicated by a variety of different non-cardiac conditions including transverse colon cancer (patient No.8), spontaneous pneumothorax (patient No.9), reflux esophagitis (patient No.11), intercostal neuralgia (patient No.12), acute leukemia (patient No.13) and idiopathic disease (patient No.10). Eight women underwent cesarean section, three delivered vaginally (patients No.8, No.10 and No.12), and two (patients No.7 and No.13) were aborted in the first trimester. Patient No.7 chose abortion in early pregnancy, 12 days after stent implantation, and was lost to follow up after discharge. Patient No.13 underwent induced abortion following two courses of chemotherapy against acute leukemia and was also lost to follow up. Patient No.2 died due to the septic shock and subsequent multi-organ dysfunction syndrome after cesarean section and aortic surgery. The remaining ten cases did not report any abnormalities during follow-up. Among the 11 neonates, seven were preterm infants. All of the 11 babies were normal in follow-ups except one preterm infant (case 8) showed growth retardation at 11 months old.ConclusionsChest pain during pregnancy may be caused by various reasons, and the severity varies greatly, and may even result in maternal and infant deaths in some circumstances. Early identification diagnosis and timely intervention are required to improve maternal and fetal outcomes.

Clinical value of MRI in treatment choice of anterior disc displacement with reduction / 中华放射学杂志

Objective To investigate the clinical value of MRI in treatment choice of anterior disc displacement with reduction. Methods 1.5 T superconducting MR was used to scan bilateral temporomandibular joints in 72 consecutive patients who were diagnosed by MRI as unilateral(66 patients)/bilateral(6 patients) anterior disc displacement with reduction at closed and open mouth. MRI sequences included oblique sagittal T2 weighted image and proton density weighted image, and 78 joints' images were acquired. According to the severity of clinical symptoms, all joints were divided into severe symptom group (45 joints) and mild symptom group (33 joints). Treatment was performed in severe symptom group , while follow up was conducted in mild symptom group. Disk position(angle between posterior margin of the disc and the condyle vertical line), disk morphology(biconcave, biplanar, biconvex, rounded, folded, thick posterior band), and joint effusion (none effusion, mild effusion, moderate effusion, marked effusion) were evaluated by two radiologists. The observer agreement for image evaluation was calculated using Kappa statistics. Group difference in disk position was compared using t-test and Chi-square test was used to compare group difference in disk morphology and joint effusion. Results The Kappa value between two radiologists were 0.816 and 0.832 (P<0.01) on evaluation of disk morphology and joint effusion. Statistical results indicated that the angles between posterior margin of the disc and the condyle vertical line in severe symptom group (54.2 ± 10.9)° were larger than those in mild symptom group (46.4 ± 9.0)° (t=3.37, P<0.05). There was no significant difference in disc deformation incidence between the two groups (χ2=1.18,P=0.277). The common deformation in sever symptom group was thick posterior band (χ2=5.65, P<0.05), and in mild symptom group was biplanar (χ2=5.67, P<0.05). No statistical difference in joint effusion incidence was observed(χ2=1.02,P=0.312). Moderate and marked effusion were more common in sever symptom group than that in mild symptom group (χ2=6.55,P<0.05). Conclusions MRI is a useful tool for making treatment choice in anterior disc displacement with reduction. Treatment should be given when the following occurred on MRI:moderate anterior disc displacement, disc deformation (excepting for biplanar), moderate or marked joint effusion.

Effect of levothyroxine combined with donepezil hydrochloride in adult rats with hypothyroidism and its mechanism / 重庆医学

Objective To investigate the effect and potential mechanism of levothyroxine combined with donepezil hydrochloride in adulthood hypothyroidism rats.Methods A total of 60 male SD rats was divided by random number table into group A,group B,group C and group D,15 rats in each group.The rats in group B,group,C and group D were treated with PLT to prepare hypothyroidism model,and group A was used as the control group.After successful preparation of the model,rats in group C and group D were injected intraperitoneally with levothyroxine(6 g/100 g) every day,and then group D was given another 0.005％ donepezil in drinking water.After 2 weeks,the learning and memory behavior of rats was observed by Morris water maze;the levels of serum T3 and T4 were measured by radioimmunoassay;the expression of syntaxin-1 in hippocampus was detected by immunohistochemistry and Western blot.Results The escape latency and swimming distance of rats were prolonged significantly in group B but shorted significantly in group C and group D,and swimming speed was significantly slowed in group B but significantly strengthened in group C and group D;the levels of T3 and T4 were obviously decreased in group B but increased significantly in group C and group D,especially in group D.The results of immunohistochemistry and Western blot showed that the expression level of syntaxin-1 in hippocampus of rats was lower in group B compared to control group but increased significantly in both group C and group D.Conclusion Levothyroxine combined with donepezil hydrochloride can improve the learning and memory ability in adult rats with hypothyroidism,and its mechanism is related to the increase of expression of synaptotagmin-1 in hippocampus.

Continuous Positive Airway Pressure Treatment for Epilepsy Complicated with Obstructive Sleep Apnea Syndrome / 天津医药

Objective To investigate the effect of continuous positive airway pressure (CPAP) on seizure frequency in epilepsy patients complicated with obstructive sleep apnea (OSA). Methods Subjects were divided into CPAP group (20 subjects) and medication group (22 subjects) according to whether they can tolerate CPAP. CPAP group were treated with CPAP combined with antiepileptic drugs. Medication group were treated with antiepileptic drugs alone. Seizure frequency and apnea-hypopnea index (AHI) were compared between groups before and after 4 weeks and 24 weeks of treatment. Results Baseline seizure frequency and apnea-hypopnea index (AHI) were compared between groups(P>0.05). A sig?nificant reduction of seizure frequency was observed in CPAP group after 4 weeks and 24 weeks of treatment compared to that before treatment (P0.05). A significant reduction of AHI was ob?served in the CPAP group after 24 weeks of treatment compared to that before treatment (P0.05). Conclusion Treatment of OSA in patients complicated with epilep?sy may improve seizure control in short and long term, but longer observation time and more samples are needed for further research.

Application research of cone beam CT in dowel preparation for the mandibular first molar / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To measure the root anatomic topography of mandibular first molar using cone beam CT (CBCT), calculate the proper space for dowel preparation.</p><p><b>METHODS</b>The roots of 121 mandibular first molars from 71 patients were scanned by CBCT, the images from cross section, sagittal plane, and coronal plane were got respectively. The dowel preparation spaces according to established three dimensional images and dowel preparation requirements were analyzed.</p><p><b>RESULTS</b>Three dowels could be prepared. The dowel length for distal-root was (7.19 +/- 0.91) mm, dowel diameter was (0.80 +/- 0.12) mm on the level of 4mm above root apex. The dowel length for mesio-root was (6.92 +/- 1.02) mm, and dowel diameter was (0.71 +/- 0.08) mm on the lever of 4 mm above root apex for buccal side and (0.71 +/- 0.07) mm for lingual side. Dowel should be prepared cone-shaped.</p><p><b>CONCLUSION</b>Many factors affect dowel preparation, individualized design is needed. CBCT can provide reliable radiographic evidence.</p>

Research of integrative medicine therapy for severe systemic lupus erythematosus from medical history analysis / 国际中医中药杂志

Objective To ivestigate integrative medicine therapy for severe systemic lupus erythematosus.Methods In 2005,we successfully salvaged a severe SLE patient,who was an inpatient from the TCM Rheumatology department of China-Japan relationship hospital.The mainly clinic manifestations included severe pulmonary hypertension,gangrene and pancytopenia.We analyzed this ease.Results By integrative medicine therapy,we successfully salvaged this patient.Conclusion The key points for integrative medicine therapy for severe systemic lupus erythematosus included:1.Using strong measures to control the disease progress,including hormones,immunosuppressive agents; 2.Positive and effective symptomatic treatment aiming at pulmonary hypertension and severe gangrene; 3.Emphasizing the effect of Chinese medicine,using integrative medicine therapy.In sum,in this case,we applied integrative medicine therapy and got very perfect clinic effect at last.

Association of plasminogen activator inhibitor-1 gene 4G/5G polymorphism and coronary heart disease in Chinese patients / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To assess whether the plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G polymorphism is associated with coronary heart disease (CHD) in Chinese patients.</p><p><b>METHODS</b>PAI-1 gene 4G /5G polymorphism was analyzed in normal group (121 individuals) and CHD group (126 cases) by a combination of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).</p><p><b>RESULTS</b>The 4G allele and 4G/4G genotype frequencies of PAI-1 gene (0.60 and 0.397) for CHD patients were higher than those (0.48 and 0.190) for healthy controls(chi-square=7.63 P<0.01; chi-square=12.67, P<0.01). The odds ratios(OR) for CHD in subjects with the 5G/5G (and 4G/5G) genotypes were 2.54 (95% CI 1.22-5.27, P<0.05) and 1.28(95% CI 1.45-2.38, P>0.05), respectively.</p><p><b>CONCLUSION</b>These results suggest that the PAI-1 4G/4G genotype is associated with an increased risk for CHD in Chinese patients. The subjects with the 4G/4G genotype had a higher prevalence of CHD, compared to those with the 5G/5G PAI-1 genotype.</p>